INDEX, COMPREHENSIVE MICROSATELLITE, AND UNIFIED LINKAGE MAPS OF HUMAN-CHROMOSOME-14 WITH CYTOGENETIC TIE POINTS AND A TELOMERE MICROSATELLITE MARKER

Three sets of linkage maps (index, comprehensive microsatellite, and u nified) have been constructed for human chromosome 14 based on genotyp es from the CEPH reference pedigrees. The index maps consist of 18 mic rosatellite markers, with heterozygosities of at least 68% and interma rker spacing no greater than 11 cM. The sex-average comprehensive micr osatellite map is 125 cM in length and includes 115 markers with 54 lo ci uniquely placed with odds for marker order of at least 1000:1. The sex-average index map length is 121 cM, and the female- and male-speci fic maps are 143 and 101 cM, respectively. A unified map was also cons tructed from 147 loci (162 marker systems), which includes 32 RFLP mar kers in addition to the 115 microsatellites. The sex-average length of the unified map is 128 cM with 69 loci uniquely placed. Our maps are anchored by a microsatellite telomere marker sCAW1 (D14S826), develope d from a telomere YAC clone TYAC196, which extends the linkage map to the physical terminus of the long arm of chromosome 14. Furthermore, w e have also physically mapped seven of the loci by fluorescence in sit u hybridization of cosmid clones or Alu-PCR products amplified from YA Cs containing the marker sequences. Together with previously establish ed cytogenetic map designations for other loci, our maps display links between genetic markers for 10 of 13 cytogenetic bands of chromosome 14 at the 550 genome band resolution. (C) 1995 Academic Press, Inc.