HUMAN GASTRIC-INHIBITORY POLYPEPTIDE RECEPTOR - CLONING OF THE GENE (GIPR) AND CDNA

Gastric inhibitory polypeptide (GIP), which is released from the gastr ointestinal tract, stimulates insulin secretion from pancreatic beta c ells and plays a crucial role in the regulation of insulin secretion d uring the postprandial phase. We have isolated the human gene (GIPR) a nd cDNA encoding the GIP receptor by a combination of the conventional screening and polymerase chain reaction procedures. Human GIP recepto r cDNA encodes a protein of 466 amino acids that is 81.5 and 81.2% ide ntical to the previously cloned hamster and rat GIP receptor, respecti vely. Hydropathic analysis shows the presence of a signal peptide and seven potential transmembrane domains, a feature characteristic of the VIP/glucagon/secretin receptor family of G protein-coupled receptors. The human GIPR gene is about 13.8 kb long, consists of 14 exons, and carries 17 Alu repeats. (C) 1995 Academic Press, Inc.