HUMAN BUCCAL EPITHELIAL-CELLS AS A POTENTIAL BIOCHEMICAL PREDICTOR OFESSENTIAL-HYPERTENSION - IDENTIFICATION OF KEY CELLULAR PROCESSES

Citation
Ej. Mcmurchie et Rj. Head, HUMAN BUCCAL EPITHELIAL-CELLS AS A POTENTIAL BIOCHEMICAL PREDICTOR OFESSENTIAL-HYPERTENSION - IDENTIFICATION OF KEY CELLULAR PROCESSES, Clinical and experimental pharmacology and physiology, 22(10), 1995, pp. 772-774
Citations number
8
Categorie Soggetti
Pharmacology & Pharmacy",Physiology
ISSN journal
03051870
Volume
22
Issue
10
Year of publication
1995
Pages
772 - 774
Database
ISI
SICI code
0305-1870(1995)22:10<772:HBEAAP>2.0.ZU;2-K
Abstract
1. Human cheek cell Na+/H+ antiporter activity (measured as the rate o f proton-dependent Na-22(+) uptake) was determined in seven normotensi ve (NT) and four hypertensive (HT) subjects following preincubation of cheek cells with a low molecular weight fraction isolated from NT sal iva together with the ionophore, nigericin. 2. Cheek cells preincubate d in this manner exhibited greater Na+/H+ antiporter activity with the mean values being 4.2 nmol Na+. mg protein 5 min for the NT group and 1.7 for the HT group. 3. It is possible that stimulation of Na+ trans port is due to cellular accumulation of K+ ions during preincubation w hich, in the presence of the K+/H+ selective ionophore, nigericin, can cause cellular reacidification promoting further Na-22(+) uptake via the Na+/H+ antiporter.