The applications of the scanning tunneling microscope (STM) in air are
usually restricted to good conducting materials as clean metals. dope
d and passivated semiconductors, or to some molecular adsorbates depos
ited onto graphite. In order to study poor conducting materials as bio
logical molecules, we have built a very low current STM. This instrume
nt can routinely be operated at 0.1 pA while having a bandwidth of 7 k
Hz. The advantages of using very low currents are illustrated by imagi
ng 5-nm-thick purple membranes. These membranes can only be imaged at
currents smaller than 2 pA. (C) 1995 American Institute of Physics.