ROLLING IN P-SELECTIN DEFICIENT MICE IS REDUCED BUT NOT ELIMINATED INTHE DORSAL SKIN

P-selectin-mediated rolling is believed to be important in the recruit ment of leukocytes to tissue after ischemia-reperfusion injury. The do rsal skin chamber was used to examine differences in the rolling and s table adhesion of circulating leukocytes in subcutaneous (SC) vessels of P-selectin-deficient and age-matched wild-type mice, both under bas al conditions and after ischemia-reperfusion. Rolling in the postcapil lary venules in SC tissue of P-selectin-deficient mice was significant ly lower than that in wild-type mice under the basal conditions and po st-ischemia-reperfusion (P < .05), but was not eliminated by the delet ion of the P-selectin gene. No significant difference between P-select in-deficient and wild-type mice in shear rate or leukocyte-endothelial adhesion was observed up to 24 hours after ischemia-reperfusion. Thes e results show that P-selectin-mediated rolling is not a prerequisite for ischemia-reperfusion-induced leukocyte-endothelial adhesion in the skin. (C) 1995 by The American Society of Hematology.