Wa. Hubert et Wa. Gern, INFLUENCE OF EMBRYONIC STAGE ON SURVIVAL OF CUTTHROAT TROUT EXPOSED TO TEMPERATURE REDUCTION, The Progressive fish-culturist, 57(4), 1995, pp. 326-328
We assessed the influence of developmental stage on the survival of em
bryos of cutthroat trout (Oncorhynchus clarki) fertilized at 7 degrees
C and subsequently cooled to 3 degrees C. Survival of embryos cooled
to 3 degrees C at or prior to 11 d after fertilization was significant
ly lower than controls held at 7 degrees C. Survival did not differ fr
om controls when embryos were cooled 13 or 15 d after fertilization. E
mbryos that had developed through neural tube closure did not show hig
her mortality than controls when cooled from 7 to 3 degrees C. Cooling
embryos to 3 degrees C after 13 or 15 d at 7 degrees C prolonged the
time to hatching by about 40 d when compared with embryos maintained a
t 7 degrees C throughout development.