CDNA EXPRESSED SEQUENCE TAGS OF TRYPANOSOMA-BRUCEI-RHODESIENSE PROVIDE NEW INSIGHTS INTO THE BIOLOGY OF THE PARASITE

A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub -library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (3 9%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 cDNAs display significant simil arities to genes in unrelated organisms encoding metabolic enzymes, si gnal transduction proteins, transcription factors, ribosomal proteins, histones, a proliferation-associated protein and thimet oligopeptidas e, among others. 313 of the cDNAs are not related to any other sequenc es in the databases. These cDNA ESTs provide new avenues of research f or exploring both the novel trypanosome-specific genes and the genome organization of this parasite, as well as a resource for identifying t rypanosome homologs to genes expressed in other organisms.