FLUORESCENCE LABELING OF MEMBRANE-BOUND CALCIUM AND CALMODULIN DURINGREGENERATION OF SOLANUM-TUBEROSUM L PROTOPLASTS

With chlorotetracycline (CTC) and fluphenazine (FFZ) respectively fluo rescence the localization of (membrane- bound) calcium and calmodulin was observed. Freshly isolated protoplasts of dihaploid Solanum tubero sum (DH 81.71463) showed a hight cytoplasmic fluorescence. Later on, w hen cell division took place, the fluorescence was localized around th e plasmatic membrane. Dividing cultures gave a strong CTC and FPZ fluo rescence during the first days of protoplasts cultivation. Cell walls, nuclei and plastids were without fluorescence according to these fact s we conclude that no specific distribution of membrane-bound calcium and activated calmodulin or rearragements of cellular calcium pools ca n be observed during cultivation of leaf mesophyll protoplasts.