MAMMALIAN HOMOLOGS OF CAENORHABDITIS-ELEGANS UNC-13 GENE DEFINE NOVELFAMILY OF C-2-DOMAIN PROTEINS

The unc-13 gene in Caenorhabditis elegans is essential for normal pres ynaptic function and encodes a large protein with C-1- and C-2-domains . In protein kinase C and synaptotagmin, C-1- and/or C-2-domains are r egulatory domains for Ca2+, phospholipids, and diacylglycerol, suggest ing a role for unc-13 in regulating neurotransmitter release. To deter mine if a similar protein is a component of the presynaptic machinery for neurotransmitter release in vertebrates, we studied unc-13 homolog ues in rat. Molecular cloning revealed that three homologues of unc-13 called Munc13-1, -13-2, and -13-3 are expressed in rat brain. Munc13s are large, brain-specific proteins with divergent N termini but conse rved C termini containing C-1- and C-2-domains. Specific antibodies de monstrated that Munc13-1 is a peripheral membrane protein that is enri ched in synaptosomes and localized to plasma membranes but absent from synaptic vesicles. Our data suggest that the function of unc-13 in C. elegans is conserved in mammals and that Munc13s act as plasma membra ne proteins in nerve terminals. The presence of C-1- and C-2-domains i n these proteins and the phenotype of the C. elegans mutants raise the possibility that Munc13s may have an essential signaling role during neurotransmitter release.