COMPARISONS OF IGE, IGG, AND IGG4 RESPONSIVENESS TO DERMATOPHAGOIDES-FARINAE IN CHILDREN BY IMMUNOBLOTTING

Although asthmatic children are often sensitized to the house-dust mit e (HDM) during early childhood, it is not clear what antigenic compone nts are associated with the early immune response of these children. T o investigate this problem, we evaluated IgE, IgG, and IgG4 antibody r esponses to Dermatophagoides farinae (Df) by immunoblotting among thre e groups of children: group I aged 0-4 years, group II aged 5-9 years, and group III aged 10-14 years. In the group I subjects, positive IgE -binding reactions to 15- and 25-kDa components were found in 76% and 44% of sera, respectively. Those to other components were generally lo w in frequency. In contrast, positive IgG-binding reactions to 15- and 25-kDa components were found in 44% and 24% of sera, and those to 30- and 110-kDa components in 48% and 88% of sera, respectively. Positive IgG4 reactions to 15- and 25-kDa components were found in 48% and 24% , respectively, and those to other components were very low. Positive IgE-binding reactions to these components gradually became more freque nt with increasing age in groups II and III, while IgG and IgG4 reacti vities were not markedly different in these age groups. These results suggest that the 15-and 25-kDa proteins of Df are important antigens a ssociated with the initial IgE response to HDM in early childhood, whi le the 30- and 110-kDa proteins are associated with IgG and 15-kDa com ponents with IgG4.