Ys. Qu et al., MODULATION OF CARDIAC NA-1 SUBUNITS( CHANNEL EXPRESSION IN XENOPUS OOCYTES BY BETA), The Journal of biological chemistry, 270(43), 1995, pp. 25696-25701
Voltage-gated Na+ channels consist of a large alpha subunit of 260 kDa
associated with beta 1 and/or beta 2 subunits of 36 and 33 kDa, respe
ctively. alpha subunits of rat cardiac Na+ channels (rH1) are function
al when expressed alone in Xenopus oocytes or mammalian cells. beta 1
subunits are present in the heart, and localization of beta 1 subunit
mRNA by in situ hybridization shows expression in the perinuclear cyto
plasm of cardiac myocytes. Coexpression of beta 1 subunits with rH1 al
pha subunits in Xenopus oocytes increases Na+ currents up to 6-fold in
a concentration dependent manner. However, no effects of beta 1 subun
it coexpression on the kinetics or voltage dependence of the rH1 Na+ c
urrent were detected. Increased expression of Na+ currents is not obse
rved when an equivalent mRNA encoding a nonfunctional mutant beta 1 su
bunit is coexpressed. Our results show that beta 1 subunits are expres
sed in cardiac muscle cells and that they interact with alpha subunits
to increase the expression of cardiac Na+ channels in Xenopus oocytes
, suggesting that beta 1 subunits are important determinants of the le
vel of excitability of cardiac myocytes in vivo.