IMMUNOCHEMICAL CHARACTERIZATION AND DEVELOPMENTAL EXPRESSION OF SHAKER POTASSIUM CHANNELS FROM THE NERVOUS-SYSTEM OF DROSOPHILA

Citation
O. Rogero et Fj. Tejedor, IMMUNOCHEMICAL CHARACTERIZATION AND DEVELOPMENTAL EXPRESSION OF SHAKER POTASSIUM CHANNELS FROM THE NERVOUS-SYSTEM OF DROSOPHILA, The Journal of biological chemistry, 270(43), 1995, pp. 25746-25751
Citations number
49
Categorie Soggetti
Biology
ISSN journal
00219258
Volume
270
Issue
43
Year of publication
1995
Pages
25746 - 25751
Database
ISI
SICI code
0021-9258(1995)270:43<25746:ICADEO>2.0.ZU;2-S
Abstract
We have raised antisera against recombinant peptides expressed from cD NAs fragments common to all splicing variants generated at the Shaker locus of Drosophila and used them as a tool to biochemically character ize these channel proteins, This antisera succeeded in detecting the e xpression of multiple Shaker potassium channels (Sh Kch), proteins wit h variable molecular mass (65-85 kDa) and pi (5.5-7), Additionally, fo r first time, specific Sh proteins of 40-45 kDa most probably correspo nding to some of the so called short Sh cDNAs previously isolated by o thers have been identified, Using genetic criteria, it has been determ ined that at least a good part of this variety of proteins is generate d by alternative splicing, Developmental experiments show a double wav e of Sh Hch channel expression with a first pick at the third instar l arvae stage, a minimum at the beginning of puparation, and the highest plateau 36 h after hatching of adult flies, The pattern of Sh splice variants changes dramatically throughout development. A detergent-resi stant fraction with about 50% of Sh Kch which seems to be anchored to submembranous structures has been found, Finally, other biochemical pr operties of Sh Kch, like membrane fractionation and glycosylation, are also described.