Kv. Reddy et al., INDUCTION OF YA(1) SUBUNIT OF RAT HEPATIC GLUTATHIONE S-TRANSFERASES BY EXERCISE-INDUCED OXIDATIVE STRESS, Archives of biochemistry and biophysics, 323(1), 1995, pp. 6-10
Adult female rats subjected to exercise training in the form of swimmi
ng daily (for 20 min initially with gradual increase up to 90 min with
in a week) for 130 days showed a significant elevation of liver glutat
hione S-transferase (GST) activity when compared to controls. Electrop
horesis and Western blot analysis of cytosolic as well as affinity-pur
ified hepatic glutathione S-transferases revealed the induction of Ya-
sized subunit in exercise-trained animals. Reverse-phase HPLC analysis
of affinity-purified GSTs further revealed that Ya-sized subunits in
control animals consisted of predominantly Ya(2), whereas the trained
animals displayed a dramatic 4.3-fold increase in Ya(1). The GSTs of e
xercise-trained animals showed increased peroxidase activity when comp
ared to corresponding controls, which is consistent with the changes i
n subunit composition. This could be a response to the physiological o
xidative stress induced by physical exercise. (C) 1995 Academic Press,
Inc.