Kc. Godowski et al., WHOLE MOUTH MICROBIOTA EFFECTS FOLLOWING SUBGINGIVAL DELIVERY OF SANGUINARIUM, Journal of periodontology, 66(10), 1995, pp. 870-877
AN INCREASED INCIDENCE OF ANTIBIOTIC-RESISTANT bacteria and yeast over
growth has been reported following various periodontal treatments. The
objective of this study was to detect possible overgrowth of opportun
istic bacteria and fungi as well as changes in normal microbiota after
application of a biodegradable delivery system containing 5% sanguina
rium (ABDS-S) to one quadrant in a split-mouth study, An oral hygiene
quadrant served as a control. The ABDS-S treated and control periodont
al sites as well as the saliva of 17 subjects were sampled prior to tr
eatment, immediately after ABDS-S removal at 7 days, and again at 30 a
nd 60 days, At Day 7 sanguinarium-resistant bacteria increased in both
control and ABDS-S periodontal sites as well as in the saliva. Enteri
c Gram-negative bacilli in both control and ABDS-S periodontal sites w
ere 2.2 to 3.4 log colony forming units higher at Day 7 compared to ba
seline, This overgrowth was transient in that levels became undetectab
le at Days 30 and 60, No such overgrowth was observed for C. albicans
or other fungi, or for S, aureus or other staphylococci in any periodo
ntal sites. Levels of Actinomyces increased at Days 30 and 60 in both
control and ABDS-S sites as well as saliva. These changes strongly sug
gest that a 7 day ABDS-S treatment in one quadrant of the mouth led to
significant microbiota changes in the treated and control quadrants a
s well as in the saliva. Future microbial studies involving antimicrob
ials delivered by local delivery systems must consider the crossover e
ffects of treatment inherent in the split-mouth design.