WHOLE MOUTH MICROBIOTA EFFECTS FOLLOWING SUBGINGIVAL DELIVERY OF SANGUINARIUM

AN INCREASED INCIDENCE OF ANTIBIOTIC-RESISTANT bacteria and yeast over growth has been reported following various periodontal treatments. The objective of this study was to detect possible overgrowth of opportun istic bacteria and fungi as well as changes in normal microbiota after application of a biodegradable delivery system containing 5% sanguina rium (ABDS-S) to one quadrant in a split-mouth study, An oral hygiene quadrant served as a control. The ABDS-S treated and control periodont al sites as well as the saliva of 17 subjects were sampled prior to tr eatment, immediately after ABDS-S removal at 7 days, and again at 30 a nd 60 days, At Day 7 sanguinarium-resistant bacteria increased in both control and ABDS-S periodontal sites as well as in the saliva. Enteri c Gram-negative bacilli in both control and ABDS-S periodontal sites w ere 2.2 to 3.4 log colony forming units higher at Day 7 compared to ba seline, This overgrowth was transient in that levels became undetectab le at Days 30 and 60, No such overgrowth was observed for C. albicans or other fungi, or for S, aureus or other staphylococci in any periodo ntal sites. Levels of Actinomyces increased at Days 30 and 60 in both control and ABDS-S sites as well as saliva. These changes strongly sug gest that a 7 day ABDS-S treatment in one quadrant of the mouth led to significant microbiota changes in the treated and control quadrants a s well as in the saliva. Future microbial studies involving antimicrob ials delivered by local delivery systems must consider the crossover e ffects of treatment inherent in the split-mouth design.