USE OF CHROMAGAR CANDIDA MEDIUM FOR ISOLATION OF YEASTS FROM DENTAL SAMPLES

A new differential medium, CHROMagar Candida, for the isolation of cli nically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on th e medium was not significantly different from the recovery on Sabourau d dextrose agar. The identities of 450 green colonies on CHROMagar Can dida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-ace tylgalactosaminidase. Candida tropicalis also formed distinctive colon ies, and other yeasts including Candida (Torulopsis) glabrata, Candida parapsilosis, Candida magnoliae, Candida lusitaniae, Candida famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a v ery useful medium, and its use will facilitate the study of yeasts ass ociated with dental diseases.