APPLICATION OF PCR FOR DETECTION OF TOXIGENIC CORYNEBACTERIUM-DIPHTHERIAE STRAINS ISOLATED DURING THE RUSSIAN DIPHTHERIA EPIDEMIC, 1990 THROUGH 1994

Authors
MIKHAILOVICH VM MELNIKOV VG MAZUROVA IK WACHSMUTH IK WENGER JD WHARTON M NAKAO H POPOVIC T
Citation
Vm. Mikhailovich et al., APPLICATION OF PCR FOR DETECTION OF TOXIGENIC CORYNEBACTERIUM-DIPHTHERIAE STRAINS ISOLATED DURING THE RUSSIAN DIPHTHERIA EPIDEMIC, 1990 THROUGH 1994, Journal of clinical microbiology, 33(11), 1995, pp. 3061-3063
Citations number
11
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
11
Year of publication
1995
Pages
3061 - 3063
Database
ISI
SICI code
0095-1137(1995)33:11<3061:AOPFDO>2.0.ZU;2-J
Abstract
A total of 250 Corynebacterium diphtheriae isolates from clinical case s and carriers in Russia were assayed by PCR directed at the A subunit of the diphtheria toxin gene to distinguish toxigenic from nontoxigen ic strains; 170 strains were positive as indicated by the presence of the 248-bp amplicon. The results of this PCR assay were in complete co ncordance with those of the standard immunoprecipitation assay (Elek), and the PCR assay is a useful tool for rapid identification in clinic al laboratories.