PROFERRIOXAMINE SYNTHESIS IN ERWINIA-AMYLOVORA IN RESPONSE TO PRECURSOR OR HYDROXYLYSINE FEEDING - METABOLIC PROFILING WITH LIQUID-CHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY

Metabolic profiling by capillary liquid chromatography-electrospray ma ss spectrometry was used to monitor shifts in the proferrioxamine prof iles of Erwinia amylovora in response to externally supplied potential proferrioxamine precursors, selected stable-isotope-labeled precursor s and atypical precursors, Based on the qualitative and quantitative s hifts in the proferrioxamine profiles, lysine and arginine are unambig uous, and agmatine, ornithine, diaminobutyric acid and the correspondi ng C-3-5 diamines are highly likely precursors for proferrioxamine bio synthesis in E, amylovora. 5-Hydroxylysine (Hyl), a recently discovere d growth inhibitor for E, amylovora, suppresses proferrioxamine produc tion. The Hyl-induced growth inhibition can be reversed by basic amino acids, The basic amino acids also partly restore proferrioxamine synt hesis.