ISOLATION AND CHARACTERIZATION OF THE SIDEROPHORE N-DEOXYSCHIZOKINEN FROM BACILLUS-MEGATERIUM ATCC-19213

N-Deoxyschizokinen, a novel siderophore, was isolated from stationary phase cultures of Bacillus megaterium ATCC 19213 and identified as pro pyl)amino]-2-oxoethyl]-2-hydroxy-4-oxo-butanoic acid. The siderophore was purified by HPLC and its structure determined using H-1 and C-13 N MR, H-1-H-1 COSY and electrospray mass spectroscopy, The monohydroxama te siderophore has the same carbon skeleton as schizokinen but the hyd roxyl group on one hydroxamate is replaced by a hydrogen, A detailed H -1 NMR study of schizokinen, N-deoxyschizokinen and their imides, schi zokinen A and N-deoxyschizokinen A is presented.