POLYMORPHIC CAC T REPETITIVE SEQUENCES IN THE PIG GENOME-1/

Citation
W. Coppieters et al., POLYMORPHIC CAC T REPETITIVE SEQUENCES IN THE PIG GENOME-1/, Animal genetics, 26(5), 1995, pp. 327-330
Citations number
12
Categorie Soggetti
Genetics & Heredity","Veterinary Sciences
Journal title
ISSN journal
02689146
Volume
26
Issue
5
Year of publication
1995
Pages
327 - 330
Database
ISI
SICI code
0268-9146(1995)26:5<327:PCTRSI>2.0.ZU;2-F
Abstract
Three genomic clones were isolated from a size-selected pig DNA librar y by hybridization with a DNA-fingerprint probe. Analysis at the seque nce level revealed that all three clones contain interrupted stretches of triplet repeats mainly composed of CAC and CAT triplets. Evaluatio n of the corresponding loci for polymorphism by Southern blot hybridiz ation showed considerable length variation. For two loci the polymorph ism was also demonstrated by polymerase chain reaction (PCR) amplifica tion, The PiGMaP reference pedigree was typed for all three loci.