Samples of milk were taken from 778 black pied cows and 368 cows of th
e Angler breed during the period from January to May 1990, and analyse
d concerning their protein fraction contents by means of HPLC. It was
found that the protein fraction contents varied considerably between c
ows. They depended on the herd level, the stage of lactation and the c
ows' age as well. The High Performance ion-Exchange Chromatography of
the major bovine milk proteins is an alternative method to the electro
phoresis with densitometry for the qualitative and quantitative analys
is of milk proteins. The successful separation and identification of w
hey proteins and caseins showed us that with this method complex biolo
gical systems could be analysed.