THE EFFECT OF KUPFFER CELL ELIMINATION ON ETHANOL-INDUCED LIVER-DAMAGE IN MICE

C57BL/10 mice develop inflammatory and necrotic changes in the liver, as well as raised serum ALT activities, after 9 days of exposure to et hanol vapour. If mice were injected twice with liposomes containing di chloromethylene diphosphonate (DMDP), with an interval of 5 days betwe en the injections, there was complete elimination of. Kupffer cells (h epatic macrophages) for a 9-day period starting 1 day after the first injection. The inflammatory and necrotic changes were significantly re duced in mice injected with liposomes containing DMDP as compared to u ninjected mice or mice injected with empty liposomes; serum ALT activi ties were also significantly reduced. No significant difference was se en in serum tumour necrosis factor-a levels between the different grou ps. Kupffer cells therefore play a significant role in the development of the liver damage resulting from exposure to ethanol. Acetaldehyde production by Kupffer cells is one way in which these cells can damage hepatocytes and further work needs to be done to investigate this and other mechanisms.