COMPARATIVE DETERMINATION OF ASCORBIC-ACID IN BASS (MORONE LEBRAX) LIVER BY HPLC AND DNPH METHODS

Authors
OTLES S
Citation
S. Otles, COMPARATIVE DETERMINATION OF ASCORBIC-ACID IN BASS (MORONE LEBRAX) LIVER BY HPLC AND DNPH METHODS, International journal of food sciences and nutrition, 46(3), 1995, pp. 229-232
Citations number
17
Categorie Soggetti
Food Science & Tenology","Nutrition & Dietetics
Journal title
International journal of food sciences and nutrition → ACNP
ISSN journal
09637486
Volume
46
Issue
3
Year of publication
1995
Pages
229 - 232
Database
ISI
SICI code
0963-7486(1995)46:3<229:CDOAIB>2.0.ZU;2-I
Abstract
A reverse-phase high pressure liquid chromatographic technique (RP-HPL C) was developed for the analysis of ascorbic acid in bass liver. Asco rbic acid was extracted from bass liver and simultaneously assayed by RP-HPLC. The results were compared to the Dinitrophenylhydrazine (DNPH ) method now in use. Recovery studies showed about 97% by the HPLC met hod compared to about 96% by the DNPH method. There was no statistical ly significant difference found in the values obtained from the two me thods. The HPLC method described here is considered the preferred meth od both in terms of a shorter analysis time and greater sensitivity.