HUMAN PAPILLOMAVIRUS AND DISEASE-FREE SURVIVAL IN FIGO STAGE IB CERVICAL-CANCER

To determine if human papillomavirus (HPV) in the primary tumor was as sociated with disease free survival of stage Ib cervical cancer patien ts, archival tissues from 47 patients were analyzed for HPV DNA by in situ hybridization (ISH) and polymerase chain reaction. HPV integratio n was determined by ISH signal pattern. Laboratory data were correlate d with clinical parameters and disease-free survival. Kaplan-Meier est imates of 4-year disease-free survival were 56% in women with HPV dete cted in the primary tumor by ISH and 100% for women in whom HPV was no t detected (P = .02). Four-year disease-free survival was 39% for pati ents with integrated HPV in the primary tumor (P = .005 vs. HPV-negati ve tumors and .05 vs. HPV episomal or episomal/integrated). HPV detect ion and integration state was not associated with any other clinical v ariable. Detection of integrated HPV DNA in the primary tumor was stro ngly associated with treatment failure.