REDUCED CONGENITAL CYTOMEGALOVIRUS (CMV) INFECTION AFTER MATERNAL IMMUNIZATION WITH A GUINEA-PIG CMV GLYCOPROTEIN BEFORE GESTATIONAL PRIMARY CMV INFECTION IN THE GUINEA-PIG MODEL
Cj. Harrison et al., REDUCED CONGENITAL CYTOMEGALOVIRUS (CMV) INFECTION AFTER MATERNAL IMMUNIZATION WITH A GUINEA-PIG CMV GLYCOPROTEIN BEFORE GESTATIONAL PRIMARY CMV INFECTION IN THE GUINEA-PIG MODEL, The Journal of infectious diseases, 172(5), 1995, pp. 1212-1220
This study evaluated the effects of subunit guinea pig (GP) cytomegalo
virus (CMV) immunization on congenital infection. Two 25-mu g doses of
an abundant GPCMV glycoprotein, the gp60-90 complex, plus adjuvant to
GPs before pregnancy produced virus-specific humoral (neutralizing an
d ELISA) and cellular (proliferative and delayed type hypersensitivity
) responses, Viral challenge before midgestation resulted in shorter m
aternal viremia in immunized than in unimmunized dams (3 vs. 17 days).
Litters of immunized dams had reduced organ involvement and rates of
congenital infection (48% vs. 18%) and increased mean birth weights (7
4 vs. 99 g). Amplification of DNA extracted from pup blood or organs w
ith primers from the gB gene of GPCMV revealed congenital infection in
some pups without detectable CMV by classic culture techniques. These
data suggest that induction of preconception immunity to CMV by vacci
ne could be useful in reducing both the incidence and severity of cong
enital CMV disease.