In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly r
epeated unit. There are 150 copies of the unit at a single locus near
the long arm telomere of one of the acrocentric chromosomes in the 14-
17 group. Two additional classes of tRNA gene-cotaining repeats have b
een isolated (defined by clones p3.1 and p3.2) that have structures re
lated to that of the 3.18 kb unit. Using in situ hybridization at the
electron microscopic level, the p3.2 repeats are found clustered at a
single locus in the subtelomeric region on one of the submetacentric c
hromosomes, whereas the p3.1 repeats are clustered at a locus indistin
guishable from that containing the 3.18 kb repeats. This suggests that
these tDNA tandem repeats can diverge in sequence from each other wit
hout being at distantly separated loci.