T. Wun et al., DESMOPRESSIN STIMULATES THE EXPRESSION OF P-SELECTIN ON HUMAN PLATELETS IN-VITRO, The Journal of laboratory and clinical medicine, 126(4), 1995, pp. 401-409
Citations number
38
Categorie Soggetti
Medical Laboratory Technology","Medicine, General & Internal
Desmopressin (1-desamino-8-D-arginine vasopressin (DDAVP)) is a synthe
tic analog of arginine vasopressin (AVP) and is useful in the treatmen
t of some bleeding disorders. The mechanism of improved hemostasis in
patients with platelet dysfunction is uncertain. Platelet-rich plasma
samples from 35 normal subjects were incubated with serial dilutions o
f DDAVP, AVP, and adenosine diphosphate. The expression of the platele
t activation-dependent antigen CD62 (P-selectin) was measured by fluor
escent-labeled monoclonal antibody and flow cytometry. DDAVP at concen
trations of 1.0 to 1000 nmol/L stimulated significant expression of CD
62 on normal platelets in vitro. At a pharmacologic concentration of D
DAVP (1 nmol/L), 14.1% (0.6% to 45.4%) (median and range) of platelets
expressed CD62. There was a strong correlation between DDAVP-induced
and AVP-induced CD62 expression (r(s) = 0.62, p = 0.0008) but not betw
een DDAVP-induced and ADP-induced expression, suggesting a V-1 recepto
r-mediated mechanism. Preincubation of platelets with a vasopressin V-
1 receptor antagonist completely inhibited CD62 expression in response
to DDAVP. We conclude that DDAVP directly activates platelets by inte
raction with the platelet V-1 receptor in vitro. This finding may part
ially explain in vivo effects of DDAVP on hemostasis.