STUDIES ON CONSTITUENTS WITH CYTOTOXIC AND CYTOSTATIC ACTIVITY OF 2 TURKISH MEDICINAL-PLANTS PHLOMIS-ARMENIACA AND SCUTELLARIA-SALVIIFOLIA

Ten known glycosidic compounds, betulalbuside A (1), 8-hydroxylioaloyl ,3-O-beta-D-glucopyranoside (2) (monoterpen glycosides), ipolamiide (3 ) (iridoid glycoside), acteoside (verbascoside) (4), leucosceptoside A (5), martynoside (6), forsythoside B (7), phlinoside B (8), phlinosid e C (9), and teucrioside (10) (phenylpropanoid glycosides) mere isolat ed from methanolic extracts of Phlomis armeniaca and Scutellaria salvi ifolia (Labiatae). Structure elucidations were carried out using H-1-, C-13-NMR and FAB-MS spectra, as well as chemical evidence. The cytoto xic and cytostatic activities of isolated compounds were investigated by the [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT ) method. Among the glycosides obtained here, caffeic acid-containing phenylpropanoid (or phenethyl alcohol, or phenylethanoid) glycosides w ere found to show activity against several kinds of cancer cells. Howe ver, they didn't affect the growth and viability of primary-cultured r at hepatocytes. Study of the structure-activity relationship indicated that ortho-dihydroxy aromatic systems of phenylpropanoid glycosides a re necessary for their cytotoxic and cytostatic activities.