DETERMINATION OF CHLORAMPHENICOL RESIDUES IN MEAT SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The results of a comparative study of chloramphenicol (CAP) determinat ion in meat, by high performance liquid chromatography are presented. A spectrophotometrical detector at 278 nm was used for measurement of CAP absorbance and subsequent quantitative determination. Two differen t mobile phases, were tested. The first phase consisted of acetonitril e - water (30:70 v/v) and the second of acetonitrile - di-ammonium hyd rogen phosphate 0.005M (25:75 v/v). The two procedures were compared b y means of the retention time and the sensitivity achieved. Recoveries of CAP from meat samples ranged between 63-79 %. The detection limits for the two procedures were 14.1 mu g(.)kg(-1) and 18.0 mu g.kg(-1) r espectively.