PRODUCTION AND CHARACTERIZATION OF OVINE GM-CSF EXPRESSED IN MAMMALIAN AND BACTERIAL-CELLS

A cDNA encoding ovine granulocyte-macrophage colony-stimulating factor (GM-CSF) was isolated and two forms of recombinant ovine GM-CSF were produced. A glycosylated form was produced in mammalian cells infected with a recombinant vaccinia virus encoding ovine GM-CSF. Recombinant ovine GM-CSF was also produced in Escherichia coli and purified by aff inity chromatography. Both forms of the protein were detected by ovine GM-CSF-specific monoclonal antibodies, and exhibited activity on ovin e bone marrow haemopoetic progenitor cells.