INVESTIGATIONS INTO THE UBIQUITOUS NATURE OF HIGH OR LOW IMMUNE RESPONSIVENESS AFTER DIVERGENT SELECTION FOR ANTIBODY-PRODUCTION IN COMMON CARP (CYPRINUS-CARPIO L)
Gf. Wiegertjes et al., INVESTIGATIONS INTO THE UBIQUITOUS NATURE OF HIGH OR LOW IMMUNE RESPONSIVENESS AFTER DIVERGENT SELECTION FOR ANTIBODY-PRODUCTION IN COMMON CARP (CYPRINUS-CARPIO L), Veterinary immunology and immunopathology, 48(3-4), 1995, pp. 355-366
This paper reports on the selection of individual carp with a high or
low antibody response, in combination with reproduction by gynogenesis
, in order to develop well-characterised inbred carp lines consisting
of practically unlimited numbers of carp with the same genotype. Two h
omozygous progenies, previously characterised as having a high or low
immune response to dinitrophenyl keyhole limpet haemocyanin (DNP-KLH),
were immunised with either a T-dependent (DNP-human serum albumin (DN
P-HSA)) or T-independent (trinitrophenyl lipopolysaccharide (TNP-LPS))
hapten-carrier complex. In comparison with the antibody response afte
r DNP-KLH immunisation, the response to DNP-HSA was observed to be hig
hly variable and did not differ between the divergently selected proge
nies. This suggests that the divergent selection for antibody producti
on to DNP-KLH has been carrier-specific. Immunisation with T-independe
nt TNP-LPS induced a very rapid response which differed between the hi
gh and low responders, and likely measured changes in the DNP-specific
precursor pool of B cells caused by the selection. A number of select
ed individuals with a high immune response to DNP-KLH were infected wi
th Trypanoplasma borreli, a haemoflagellate parasite of carp, to exami
ne a possible relationship between the increase in immune responsivene
ss and disease resistance, but no change could be detected. However, i
ndividual homozygous carp were able to escape inbreeding depression an
d survive the infection. Such carp would be likely candidates for gyno
genetic reproduction to obtain viable inbred carp lines.