DIFFERENTIAL ADHESION OF METASTATIC RAW117 LARGE-CELL LYMPHOMA-CELLS UNDER STATIC OR HYDRODYNAMIC CONDITIONS - ROLE OF INTEGRIN ALPHA(V)BETA(3)

RGD-containing substrates were used to study static and hydrodynamic a dhesion of murine RAW117 large-cell lymphoma sublines with differentia l liver-metastatic potentials, Highly liver-metastatic RAW117-H10 cell s had higher rates of static adhesion to vitronectin, fibronectin and (GRGDS)(4) than poorly metastatic RAW117-P and moderately liver-metast atic RAW117-L17 cells, Under hydrodynamic conditions, adhesion stabili zation was more rapid for H10 cells compared to P or L17 cells, Among the RGD peptides, only the polymeric RGD peptide (GRGDS)(4) mediated s trong static adhesion of H10 cells, Interestingly, all the RGD peptide s mediated adhesion stabilization for H10 cells but still not for L17 or P cells under hydrodynamic conditions, Integrin alpha(v) beta(3) wa s involved in stabilizing hydrodynamic adhesion to (GRGDS)(4), monomer ic RGD peptide R1, but was less important in static adhesion to monome ric RGD peptides, Differential adhesion to liver sinusoidal endothelia l cell-derived extracellular matrix (H10 much greater than L17 > P) wa s observed under hydrodynamic but not static conditions, Integrin alph a(v) beta(3) was also important in hydrodynamic adhesion to liver sinu soidal endothelial cell-derived extracellular matrix, We believe that strong static and hydrodynamic adhesion of H10 cells and their capabil ity of altering adhesive behavior in response to fluid shear may contr ibute to liver metastasis.