CHARACTERIZATION OF MULTIPLE PROHORMONE CONVERTASE PC1 3 TRANSCRIPTS IN PORCINE OVARY/

Overlapping cDNAs encoding porcine prohormone convertase, PC1/3, have been isolated from a pregnant sow ovary cDNA library using a mouse PC1 /3 cDNA as a probe. Nucleotide sequence analysis of these cDNAs predic ts a PC1/3 precursor protein of 753 amino acid residues, which shares an overall sequence homology of 96, 92, and 92% with the human, rat, a nd mouse counterparts, respectively. Furthermore, five different polya denylation sites have been observed. The utilization of these polyaden ylation sites results in a length difference of 40-440 bp in the 3' un translated regions of the transcripts.