DNA METHYLASE ACTIVITY IN HUMAN MYOCARDIUM IS ASSOCIATED WITH ACTIN PROTEINS

Authors
LOPATINA NG NIKOLSKAYA II KOVALEV LI SHARKOVA EV ERSHOVA EL KIRKEL AZ SHISHKIN SS
Citation
Ng. Lopatina et al., DNA METHYLASE ACTIVITY IN HUMAN MYOCARDIUM IS ASSOCIATED WITH ACTIN PROTEINS, Molecular biology, 29(4), 1995, pp. 513-518
Citations number
28
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
29
Issue
4
Year of publication
1995
Part
2
Pages
513 - 518
Database
ISI
SICI code
0026-8933(1995)29:4<513:DMAIHM>2.0.ZU;2-P
Abstract
Electrofocusing of human myocardial nuclear proteins yielded five peak s of DNA methylase activity in the pH range 3.5-8.2. One peak was reso lved by two-dimensional electrophoresis into six components of 10, 25, 35, 43, 67; and 120K; the 43K species corresponded in mobility to act ins. This fraction exhibited DNA(cytosine) methyltransferase activity. Sequence comparison between human actins and bacterial DNA methylases revealed extended regions of homology in their C-terminal parts.