TRYPANOSOMA-BRUCEI - IDENTIFICATION OF AN INTERNAL REGION OF PHOSPHOGLYCERATE KINASE REQUIRED FOR TARGETING TO GLYCOSOMAL MICROBODIES

Citation
Gc. Peterson et al., TRYPANOSOMA-BRUCEI - IDENTIFICATION OF AN INTERNAL REGION OF PHOSPHOGLYCERATE KINASE REQUIRED FOR TARGETING TO GLYCOSOMAL MICROBODIES, Experimental parasitology, 85(1), 1997, pp. 16-23
Citations number
27
Categorie Soggetti
Parasitiology
Journal title
ISSN journal
00144894
Volume
85
Issue
1
Year of publication
1997
Pages
16 - 23
Database
ISI
SICI code
0014-4894(1997)85:1<16:T-IOAI>2.0.ZU;2-5
Abstract
Among the microbodies found in eukaryotes are the glycosomes of Trypan osoma brucei, thought to be closely related to peroxisomes. Two types of targeting signals for glycosomes have been identified thus far: typ e 1 at the C-terminus and type 2 at the N-terminus. In this report, we use an epitope-tagging system to characterize the targeting signal fo und on the minor glycosomal isozyme of phosphoglycerate kinase, 56PGK. No type 1 or 2 signal was found; rather, the topogenic information wa s found to be internal. Chimeric molecules formed with the cytoplasmic phosphoglycerate kinase isozyme indicate that a region between amino acids 24 and 91 of 56PGK is essential for glycosomal targeting. No hom ology was found between this region and peroxisomal proteins containin g internal targeting signals. (C) 1997 Academic Press.