SPECIES-RELATED AND GENDER-RELATED DIFFERENCES IN AMINE, ALCOHOL AND PHENOL SULPHOCONJUGATIONS

1. Species-, gender- and strain-related differences in amine sulphocon jugations mere studied in 105000g supernatants of liver samples isolat ed from mouse, rat, guinea pig, rabbit, dog, monkey and man and were c ompared with those of alcoholic and phenolic compounds. Substrates exa mined were desipramine (an alkylamine), piperazine and piperidine deri vatives (alicyclic amines), aniline (an arylamine), triaramide and deh ydroepiandrosterone (alcoholic compounds) and 2-naphthol (a phenolic c ompound). 2. Sulphoconjugating activities of alicyclic and aryl-amines and tiaramide varied depending on the animal species, sex and strain used. In all animal species examined, the activity for desipramine was low or negligible but for 2-naphthol was consistently detected and hi gh. Amine sulphoconjugations were higher in rabbit than in other anima l species. Dog hepatic 105000g supernatants exhibited low or neglible activities for amines and tiaramide. Females showed higher sulphoconju gating activities for all substrates in mouse and for amines and tiara mide in rat; males exhibited higher activities for 2-naphthol in rat a nd monkey and for amines in rabbit; there were no clear sex-related di fferences in other sulphoconjugations. 3. Among BALB/c, C57BL/6, DBA/2 , and AKR mouse strains, the AKR strain showed higher activities towar ds amines and tiaramide than others. 4. In human liver 105000g superna tants, sulphoconjugating activities for alicyclic amines, dehydroepian drosterone, and 2-naphthol were detected. Among them, higher activitie s were observd in piperazine and phenol sulphoconjugations. There were no sex-related differences in the activities of all substrates examin ed. Good correlations were observed in activities between alicyclic am ine and dehydroepiandrosterone sulphoconjugations. 5. These results in dicate that activities of amine and alcohol sulphoconjugations vary co nsiderably depending on the substrate, species, sex and strain but phe nol sulphoconjugation is consistently detected in all species examined