The effects of different proteins (pork, egg albumin, egg yolk, soybea
n and casein) on iron absorption and intestinal solubility were examin
ed in growing rats. Rats were fed iron-adequate diets including one of
the protein sources for 14 d. All the diets were adjusted to contain
similar levels of protein (220 g crude protein /kg diet) and iron (51-
53 mg iron /kg diet). Apparent iron absorption was determined using co
nventional chemical balances. Soluble iron in the intestinal contents
and plasma iron concentration in portal blood were determined shortly
after the meal. Portal blood iron and intestinal soluble iron were hig
hly correlated (r = 0.99, p =0.042). The addition of pork protein grea
tly improved the solubility (42%) and apparent absorption (42%) of iro
n. In contrast, in rats fed egg yolk protein, iron solubility (17%) in
the small intestine significantly decreased together with apparent ir
on absorption (13%) and iron concentration of the portal blood when co
mpared with other groups. The effects of proteins on intestinal solubi
lity and apparent absorption of iron were in increasing order: egg yol
k < soybean < egg albumin < casein < pork. Our results suggest that th
e iron enhancing/inhibiting effect of pork/egg yolk results from their
protein fractions. The iron solubility in the small intestine may be
responsible for the difference in iron absorption of various proteins.