PHYSICAL MAPPING OF DIFFERENCES BETWEEN THE CHLOROPLAST DNAS OF BETA-VULGARIS AND B-WEBBIANA

A physical map of Beta webbiana (a wild species of the section Procumb entes) chloroplast genome was constructed by localizing the cleavage s ites of SmaI, PstI, PvuII, XhoI, and HindIII, and the map was then ali gned with the map of sugarbeet (B. vulgaris) chloroplast DNA. This ali gnment shows 27 restriction-site changes and 11 insertions/deletions, most of which occur in the large single-copy region of the genome. A 0 .7-kb long mutation,located within an unidentified open reading frame (ORF2280) in the inverted repeat, was also found.