Discordant molecular clock estimates of the divergence time between mo
nocots and dicots (angiosperms) have accumulated in recent years. The
diverse estimates are more than 100 Myr apart and are derived from nuc
lear and chloroplast gene sequences. Using two landmark events and nuc
leotide sequences from 10 mitochondrial genes for which rate homogenei
ty was formally tested, we have estimated this date to be around 200 M
yr. The estimates obtained with the two multiple-gene clocks are highl
y congruent and in line with previous estimates derived from protein-c
oding gene sequences from the chloroplast genome. The agreement of thi
s date with recent findings from the fossil record is discussed briefl
y.