This study evaluated the value of microcomputed tomography (MCT) for u
se in endodontic research. Four periodontally involved highly calcifie
d maxillary first molars were extracted and then scanned for evaluatio
n by a MCT system. The teeth were then instrumented, and 2 of the 4 ob
turated before rescanning for comparison evaluation. Several capabilit
ies of the MCT to advance endodontic research significantly were obser
ved: the ability of the MCT to present accurately the external and int
ernal morphologies of the tooth without tooth destruction; the possibi
lity of showing changes over time in surface areas and volumes of tiss
ues; the ability to assess area and volume changes after instrumentati
on or obturation; and the capability of evaluating canal transportatio
n following instrumentation or instrumentation and obturation. The tre
mendous potential of this scientific tool was discussed.