R. Tyagi et Mn. Gupta, PURIFICATION AND IMMOBILIZATION OF ASPERGILLUS-NIGER PECTINASE ON MAGNETIC LATEX BEADS, Biocatalysis and biotransformation, 12(4), 1995, pp. 293-298
A commercial preparation of pectinase pectinex was purified with the h
elp of alginate-magnetite beads. The purified pectinase was immobilize
d an magnetic latex beads via carbodiimide coupling. The maximum effic
iency of the immobilized preparation was calculated as 81% of the tota
l activity bound to the beads. This was at the lowest level of couplin
g tried. The pH optimum (pH 4.5 for both free as well as immobilized e
nzyme) and k(m) (0.7 mg/ml for free enzyme; 1 mg/ml for immobilized en
zyme) did not vary significantly upon immobilization. While the half l
ife of free enzyme was calculated as 9 min., the immobilized preparati
on remained stable up to 3 h at 60 degrees C.