PURIFICATION OF HUMAN BRAIN ENOLASE AND PHOSPHOGLYCERATE MUTASE AND FORMATION OF THEIR BIENZYMATIC COMPLEX

Authors
NAZARYAN KB PEREZ P KAZARYAN BA PEREZ P CLIMENT F
Citation
Kb. Nazaryan et al., PURIFICATION OF HUMAN BRAIN ENOLASE AND PHOSPHOGLYCERATE MUTASE AND FORMATION OF THEIR BIENZYMATIC COMPLEX, Biochemistry, 60(5), 1995, pp. 559-564
Citations number
35
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
60
Issue
5
Year of publication
1995
Pages
559 - 564
Database
ISI
SICI code
0006-2979(1995)60:5<559:POHBEA>2.0.ZU;2-3
Abstract
Neuron-specific enolase and phosphoglycerate mutase with specific acti vities of 106 and 215 units per mg, respectively, have been purified f rom human brain. Hydrophobic chromatography on octyl-Sepharose for eno lase and affinity chromatography on blue Sepharose for phosphoglycerat e mutase were used as the last steps of purification. A hetero-bifunct ional complex with fully preserved enolase and phosphoglycerate mutase activities was synthesized using a bifunctional reagent, N-succinimid yl-3-(2-pyridyldithio)propionate.