GINKGOLIDE-B PRODUCTION IN CULTURED-CELLS DERIVED FROM GINKGO-BILOBA L LEAVES

Callus cultures and cell suspension cultures derived from Ginkgo bilob a L. leaves produced ginkgolide B. In cell suspension cultures, the pr oduction reached a maximum by the 13th day of subculture and followed by a sharp decrease. The medium of Murashige and Skoog induced the hig hest ginkgolide B content in cultures while the medium of Schenk and H ildebrandt promoted cell growth. For the maximal production of ginkgol ide B, cells were cultured in Murashige and Skoog medium modified to c ontain 1.0 mg/l of alpha-naphthaleneacetic acid, 0.1 mg/l of kinetin, 30 g/l sucrose and 1.25 mM potassium phosphate with a molar ratio of a mmonium to nitrate ions of 1 : 3.