CHOLINERGIC DIFFERENTIATION FACTOR LEUKEMIA INHIBITORY FACTOR (CDF LIF) INCREASES CHOLINERGIC BUT NOT ENKEPHALIN-IMMUNOREACTIVE CELLS IN ADRENAL-MEDULLA GRAFTED TO THE RAT HIPPOCAMPUS/
M. Jousselinhosaja et al., CHOLINERGIC DIFFERENTIATION FACTOR LEUKEMIA INHIBITORY FACTOR (CDF LIF) INCREASES CHOLINERGIC BUT NOT ENKEPHALIN-IMMUNOREACTIVE CELLS IN ADRENAL-MEDULLA GRAFTED TO THE RAT HIPPOCAMPUS/, Neuroscience research communications, 17(1), 1995, pp. 1-7
The effects of Cholinergic Differentiation Factor/Leukemia Inhibitory
Factor (CDF/LIF) on the transmitter phenotype was investigated in the
chromaffin cells of adrenal medulla grafts implanted in the hippocampu
s of adult rats. In the grafted rats treated once or twice with CDF/LI
F, the evolution of the chromaffin cells from a catecholaminergic to a
cholinergic phenotypes was facilitated. The number of chromaffin cell
s immunoreactive to choline acetyltransferase (ChAT) was significantly
higher in CDF/LIF-treated than control rat grafts, a parallel signifi
cant decrease occurred in the number of chromaffin cells immunoreactiv
e to phenylethanolamine-N methyltransferase (PNMT). However, the numbe
r of chromaffin cells immunoreactive to the octapeptide M(5)-Arg-Phe-G
ly (MetEnk 8) was not significantly different in grafts from LIF-treat
ed and control rats, suggesting that LIF had no effects on the synthes
is of this octapeptide. The fact that LIF facilitates the acquisition
of cholinergic phenotypes by chromaffin cells in adrenal medulla graft
s implanted in an adult brain, may have important therapeutic applicat
ion.