When the root canals are instrumented during endodontic therapy, a lay
er of material composed of dentine, remnants of pulp tissue and odonto
blastic processes, and sometimes bacteria, is always formed on the can
al walls. This layer has been called the smear layer. It has an amorph
ous, irregular and granular appearance under the scanning electron mic
roscope. The advantages and disadvantages of the prescence of smear la
yer, and whether it should be removed or not from the instrumented roo
t canals, are still controversial. It has been shown that this layer i
s not a complete barrier to bacteria and it delays but does not abolis
h the action of endodontic disinfectants. Endodontic smear layer also
acts as a physical barrier interfering with adhesion and penetration o
f sealers into dentinal tubules, in turn, it may affect the sealing ef
ficiency of root canal obturation. When it is not removed, the durabil
ity of the apical and coronal seal should be evaluated over a long per
iod. If smear layer is to be removed, EDTA and NaOCl solutions have be
en shown to be effective, among various irrigation solutions and techn
iques, including ultrasonics, that have been tested. Once this layer i
s removed, it should be borne in mind that there is a risk of reinfect
ing dentinal tubules if the seal fails. Further studies are needed to
establish the clinical importance of the absence or presence of smear
layer.