A PLATE ASSAY FOR THE DETECTION OF ORGANOPHOSPHONATE MINERALIZATION BY ENVIRONMENTAL BACTERIA, AND ITS MODIFICATION AS AN ACTIVITY STAIN FOR IDENTIFICATION OF THE CARBON-PHOSPHORUS BOND-CLEAVAGE ENZYME PHOSPHONOACETATE HYDROLASE

Authors
MCGRATH JW QUINN JP
Citation
Jw. Mcgrath et Jp. Quinn, A PLATE ASSAY FOR THE DETECTION OF ORGANOPHOSPHONATE MINERALIZATION BY ENVIRONMENTAL BACTERIA, AND ITS MODIFICATION AS AN ACTIVITY STAIN FOR IDENTIFICATION OF THE CARBON-PHOSPHORUS BOND-CLEAVAGE ENZYME PHOSPHONOACETATE HYDROLASE, Biotechnology techniques, 9(7), 1995, pp. 497-502
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
7
Year of publication
1995
Pages
497 - 502
Database
ISI
SICI code
0951-208X(1995)9:7<497:APAFTD>2.0.ZU;2-Y
Abstract
A replica plate screening technique, based on the acid molybdate assay for detection of phosphate has been developed to permit the detection of microorganisms capable of mineralizing organophosphonates. The met hod was further adapted as the basis of an activity stain for the dete ction of the carbon - phosphorus bond cleavage enzyme phosphonoacetate hydrolase in PAGE gels.