This paper describes the genetic transformation of the astaxanthin-pro
ducing yeast Phaffia rhodozyma with the cloning vector pGH-1. The plas
mid replicates autonomously in this yeast, and the selection of transf
ormants was possible by using both, the URA3 marker from Saccharomyces
cerevisiae, and the kanamycin resistance (Km(R)) determinant from the
bacterial transposon Tn903.