SELECTIVE RELEASE OF SUPEROXIDE-DISMUTASE FROM SACCHAROMYCES-CEREVISIAE WITH ISOPROPANOL

Authors
TAN TW YANG YZ MA RY QI YZ MO XR
Citation
Tw. Tan et al., SELECTIVE RELEASE OF SUPEROXIDE-DISMUTASE FROM SACCHAROMYCES-CEREVISIAE WITH ISOPROPANOL, Biotechnology techniques, 9(7), 1995, pp. 539-542
Citations number
6
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
7
Year of publication
1995
Pages
539 - 542
Database
ISI
SICI code
0951-208X(1995)9:7<539:SROSFS>2.0.ZU;2-F
Abstract
A new and inexpensive approach by which the release and purification o f intracellular protein can be carried out simultaneously has been dev eloped. Alcoholic solvents were used to selectively release superoxide dismutase (SOD) from Saccharomyces cerevisiae. 90%(v / v) isopropanol was used to treat the cells for 120 minutes and then 50 mM phosphate buffer(pH7.0) was added after removal of the solvent by filtration. Th e recovery of SOD was up to 90%, while the specific activity of SOD in creased 25 times compared to traditional methods such as ultrasonic me thod.