CYTOGENETIC STUDY OF CALLI AND REGENERATE D CLONES IN POTATO CV BF15 (2X) (H1)

Somaclonal variation was observed on callus and regenerated clones ind uced in vitro from internodal cuttings prelevated on a dihaploid potat o clone. A preliminary study on nuclear DNA mass showed variations ind icating a genome instability increasing rapidly during the first twelv e days of culture, then decreasing with callus aging, but also increas e soon after subculture. The effect of these variations on chromosome number were analyzed on 44 regenerated clones. Finally, observations o n meiosis allowed us to sort some diploid regenerated clones with a mo re regular meiosis than the control.