In the molecular scheme of living organisms, adenosine 3',5'-monophosp
hate (cyclic AMP or cAMP) has been a universal second messenger. In eu
karyotic cells, the primary receptors for cAMP are the regulatory subu
nits of cAMP-dependent protein kinase. The crystal structure of a 1-91
deletion mutant of the type I alpha regulatory subunit was refined to
2.8 Angstrom resolution. Each of the two tandem cAMP binding domains
provides an extensive network of hydrogen bonds that buries the cyclic
phosphate and the ribose between two beta strands that are linked by
a short alpha helix. Each adenine base stacks against an aromatic ring
that lies outside the beta barrel. This structure provides a molecula
r basis for understanding how cAMP binds cooperatively to its receptor
protein, thus mediating activation of the kinase.