A combined laboratory and field study examined the potential for a sym
biotic association between the actinorhizal shrub Myrica cerifera and
vesicular arbuscular mycorrhizal (VAM) fungi on a Virginia barrier isl
and. M. cerifera seedlings and two test species, Zea mays and Strophos
tyles umbellata, were grown in an environmental chamber on soils colle
cted from four sites differing in soil age (<5 to over 130 years), sal
inity (1-35 mu g/g total soil chloride), and edaphic characteristics.
Seedling root infection was significantly lower for all three species
in the youngest soils from the beach where salinity was highest. Stain
ed M. cerifera roots revealed all the components for a functional VAM
association; however, there were significantly fewer arbuscules and ve
sicles relative to the test species. Among field-collected M. cerifera
, infection was not detected in mature shrubs from the bay side of the
island, where M. cerifera thickets were in a state of degeneration. I
nfection was highest in soils from the young, developing thickets, and
in the most stable thickets of the island interior. Despite the dynam
ic nature of the barrier island environment, VAM associations with M.
cerifera appear to be present, especially in seedlings and developing
shrub thickets.